Regulatory
Part:BBa_J100305:Design
Designed by: Sabrina Shepherd Group: Campbell M Lab (2016-09-08)
upp Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We chose our sequence regions based off of where we thought the TATA box lied between the -35 to -10 regions before the start codon.
Source
This promoter came from E. Coli and this journal article, "Regulation of upp Expression in Escherichia coli by UTP Sensitive Selection of Transcriptional Start Sites Coupled with UTP-Dependent Reiterative Transcription" by ANH-HUE THI TU AND CHARLES L. TURNBOUGH, JR. in the Journal of Bacteriology. We got this sequence from the given information about the UPP gene sequence promoter in this article and online at this web resource: http://margalit.huji.ac.il/promec/prom.seq.final.html.