Regulatory

Part:BBa_J100305:Design

Designed by: Sabrina Shepherd   Group: Campbell M Lab   (2016-09-08)


upp Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose our sequence regions based off of where we thought the TATA box lied between the -35 to -10 regions before the start codon.

Source

This promoter came from E. Coli and this journal article, "Regulation of upp Expression in Escherichia coli by UTP Sensitive Selection of Transcriptional Start Sites Coupled with UTP-Dependent Reiterative Transcription" by ANH-HUE THI TU AND CHARLES L. TURNBOUGH, JR. in the Journal of Bacteriology. We got this sequence from the given information about the UPP gene sequence promoter in this article and online at this web resource: http://margalit.huji.ac.il/promec/prom.seq.final.html.

References